Basic Study

Efficacy study of microwave in a human H322 lung cancer mouse model

We investigated the efficacy of microwave by suppressing tumor growth in a human NSCLC H322 utilizing orthotopic lung cancer mouse moodel. To establish orthotopic pleural tumors, mice were inoculated with H322 cells by previously established intrathoracic injection procedures. The mice were randomly divided into 3 groups (8 mice per group), control group (no treatment), mild (39°C) hyperthermia group, and moderate (43°C) hyperthermia group. The mice  were treated 6 times in total with microwave over 2 weeks after tumor inoculation. On day 28 after tumor inoculation, we sacrified all mice and observed the thoracic cavity in control mice. Apoptosis in frozen tumor tissue sections was detected using an In situ Cell Death Detection kit by TUNEL staining (TUNEL, bright green) and examined under a fluorescence microscope equipped with a digital camera, and a nuclear staining DAPI (blue) in the same tissue sections were also shown. Magnifications, x 400.
     Induction of apoptosis in the tumors, as indicated by the bright green fluorescein TUNEL staining, was detected in mice irradiated with microwave, but not in control mice. A much higher degree of apoptosis induction was registered in tumors irradiated and intensified TUNEL staining. Total number and weight of tumor inside the chest cavity are shown in Graph below. (Results from experiments conducted by Dr. Motomura Tadashi, Associate Professor, Baylor College of Medicine, United States).