Basic Study

Influences of microwave treatment on the Bcl-2 family of proteins by Western blotting

We examined whether the microwave hyperthermia treatment influences the Bcl-2 family of proteins by Western blotting. This treatment increased the expression of the dimer, but not the monomer form of BNIP3, whereas control group did not show any influence on either form of BNIP3. Most of the Bcl-2 family proteins such as BNIP3 work with dimer formation. Moreover, apoptotic or antiapoptotuc Bcl-2 family member Bax or Bcl-XL increased or decreased in temperature-dependent manner. Increased activation of caspase-3 and protease PARP was clearly detected in H322 cells treated with the microwave hyperthermia, compared to non-treated control group. These results suggest that the strong induction of apoptosis in cells treated with the microwave hyperthermia, are mediated both by the activation of caspase cascade and apoptotic regulation of Bcl-2 family. (Results from experiments conducted by Dr. Motomura Tadashi, Associate Professor, Baylor College of Medicine, United States).