Basic Study

The inhibitory effects of microwave irradiation on LoVo cell growth and LoVo cell DNA fragmentation

Mice were fixed in between two microwave generator units placed at a distance of 60 cm from each other, and microwaves were irradiated simultaneously on the front and back of each mouse 2 times for 3 seconds each time at 3-second intervals.  The DNA fragmentation of LoVo cells was examined 3 days after the microwave irradiation.  Fig. 1A shows the results of analysis of DNA obtained from the LoVo cells of the experimental and control groups.  As this figure shows, DNA fragmentation of LoVo cells was clearly observed in the specimens taken on day 0 (column 2) and on day 3 (column 1), after the microwave treatment. On the other hand, DNA fragmentation was not observed in the DNA analytic image (Fig. 1B) of the W1-38 cells, a normal fibroblast cell strain derived from a human fetal lung. These results indicate that microwave irradiation induces apoptosis in LoVo cells.
     Fig. 2 shows the growth inhibitory effect of the microwave treatment on LoVo cells.  In order to compare respective cell viability, a comparative study was conducted on the group treated with  2 – 4GHz microwave irradiation on LoVo cells and the untreated group.   As shown in Fig. 2, the number of living LoVo cells in the treated group decreased from 105 to an almost undetectable level after the microwave treatment, whereas the number of living cells in the untreated group increased to 3 x 105 in three days.  The results show that microwave significantly inhibited LoVo cell growth.

 

Fig. 1 Result of DNA analysis
‘A’ shows the DNA fragmentation of LoVo cells immediately after the microwave irradiation (line 2) and 3 days after the microwave irradiation (line 1). Clear fragmentation was observed 3 days after the irradiation.
‘B’ shows the DNA analytic image of the W1-38 cells, a normal fibroblast cell strain derived from a human fetal lung. No DNA fragmentation was observed.

 

Fig. 2 Inhibition of LoVo cell growth after microwave treatment. Cell growth inhibiting effect with outstanding results was observed in the group treated with microwave irradiation.